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Journal / Biology / Gene Svällning Använda Gal4 i Zebrafish
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Gene Svällning Använda Gal4 i Zebrafish

Article DOI: 10.3791/50113 • 13:34 min
September 29th, 2013 •
1, 1
1Department of Biology, Temple University

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September 29th, 2013

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Click here for the English version.

Detta protokoll beskriver metoden för genfälle insertionsmutationer använder Gal4-VP16 som den primära reporter och GFP / RFP som sekundära reportrar i zebrafisk. Ungefär en av tio högt uttrycker F0 fisk avkastnings genfälla avkomma co-uttrycker GFP och RFP. Screeningen kan lätt skalas för att anpassa sig till storleken på det laboratorium som utför insertionsmutationer skärmen.

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