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JoVE Journal Medicine
O uso de matrizes de fase reversa Proteínas (RPPA) para explorar variação Protein Expression individuais dentro de câncer de células renais
 
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O uso de matrizes de fase reversa Proteínas (RPPA) para explorar variação Protein Expression individuais dentro de câncer de células renais

Article doi: 10.3791/50221
January 22nd, 2013 •
1, 1, 1, 2, 3, 4, 4, 1,5, 6, 7, 1,2, 1
1Edinburgh Urological Cancer Group, University of Edinburgh, 2School of Medicine, University of St Andrews, 3Division of Pathology, University of Edinburgh, 4MRC Human Genetics Unit, MRC IGMM, University of Edinburgh, 5Department of Pathology, Western General Hospital, 6Breakthrough Breast Cancer Research Unit, University of Edinburgh, 7St Bartholomew's Cancer Institute, Experimental Cancer Medicine Centre, Queen Mary University of London

Summary
Automatic Translation
January 22nd, 2013

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RPPA permite a expressão da proteína de centenas de amostras, impressos em lâminas de nitrocelulose para serem interrogados ao mesmo tempo, utilizando anticorpos marcados com fluorescência. Esta técnica tem sido utilizada para estudar o efeito do tratamento com medicamentos na heterogeneidade claro carcinoma de células renais.

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