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Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

JoVE Journal
Chemistry

This content is Free Access.

JoVE Journal Chemistry

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

DOI:

10.3791/50379-v

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14:22 min

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April 15, 2013

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Chryssostomos Chatgilialoglu, Carla Ferreri, Annalisa Masi, Michele Melchiorre, Anna Sansone, Michael A. Terzidis, Armida Torreggiani

¹ISOF – Bio Free Radicals,Consiglio Nazionale delle Ricerche

Chapters

00:05Title
03:05Synthesis of Mono-trans Isomers of Cholesteryl Esters
04:34Isolation of Cholesteryl Esters Fraction from Human Serum
06:25Synthesis of (5’R)- and (5’S)-5′,8-cyclo-2′-deoxyadenosine
08:02Synthesis of (5’R)- and (5’S)-5′,8-cyclo-2′-deoxyguanosine
08:50Gamma Radiolysis of DNA Aqueous Solutions
09:38Enzymatic Digestion of DNA
10:43Results: Development of Biomarkers for Detection of Free Radical Stress
13:28Conclusion

Summary

Automatic Translation

Radical-based biomimetic chemistry has been applied to building-up libraries necessary for biomarker development.

Tags

Free Radicals Chemical Biology Biomarkers Lipids Nucleic Acids Proteins Cholesteryl Esters Cyclo-2′-deoxyribonucleosides Ionizing Radiation Health Conditions

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