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におけるプロスタグランジンの抽出と分析<em>線虫（Caenorhabditis elegans）</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Prostaglandin Extraction and Analysis in
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
におけるプロスタグランジンの抽出と分析<em>線虫（Caenorhabditis elegans）</em
DOI:
10.3791/50447-v
•
08:50 min
•
June 25, 2013
•
Jeevan K. Prasain
,
Hieu D. Hoang
,
Johnathan W. Edmonds
,
Michael A. Miller
1
Department of Pharmacology and Toxicology
,
University of Alabama at Birmingham
,
2
Department of Cell, Developmental, and Integrative Biology
,
University of Alabama at Birmingham
Chapters
00:05
Title
01:05
Worm Culture
03:28
Prostaglandin Extraction
06:14
Prostaglandin Analysis
07:15
Results: Prostaglandin Extraction and Analysis in C. elegans
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本稿では、プロスタグランジンや他のエイコサノイドからを抽出し、分析するために最適化された手順を説明<em> C.エレガンス</em> LC-MS/MSを用いた。
Tags
Prostaglandin
Caenorhabditis Elegans
Eicosanoid
Lipid Extraction
Liquid Chromatography-mass Spectrometry
Tandem Mass Spectrometry
Multiple Reaction Monitoring
Collision-induced Decomposition
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試験管内で
活性化
線虫（Caenorhabditis elegans）
精子
固体プレートベースの食餌制限
線虫（Caenorhabditis elegans）</em
の初期発生のタイムラプス顕微鏡
線虫（Caenorhabditis elegans）
基本的な<em>線虫Caenorhabditis elegans</em>方法：同期との観測
の栽培<em>線虫（Caenorhabditis elegans）</em>ラボにおける三次元で
結合されたヌクレオチドおよび
線虫
のタンパク質抽出
そのまま
線虫
の酸素消費量の測定
細胞外小胞体の
カエノールハブディティス・エレガンス
の精製と分析
カエノハブディティス・エレガン
スにおける組織特異的プロテオスタティック低下の定量化
カエノハブディティス・エレガン
スにおける年齢関連神経変性疾患のモデル化
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