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JoVE Journal Bioengineering
En forbedret metode til forberedelse af type I kollagen fra hud
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Flapping Soft Fin Deformation Modeling using Planar Laser-Induced Fluorescence Imaging
Journal (Bioengineering)
Flapping Soft Fin Deformation Modeling using Planar Laser-Induced Fluorescence Imaging
 
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En forbedret metode til forberedelse af type I kollagen fra hud

Article DOI: 10.3791/51011-v • 05:17 min • January 21st, 2014
January 21st, 2014 •
1,2, 1, 1,2
1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

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Traditionelle metoder til isolering af opløselige type 1 kollagen (Kol1) kræver omkring 10 dage fra start til slut på grund af langvarige buffer inkubationer og møjsommelig resuspensions af fibriller. Her beskriver vi et middel til at rense Kol1 fra små dermale biopsier i mindre end 3 timer.

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Bioteknik type 1 kollagen ekstracellulære matrix tissue engineering stillads protein dermis læderhuden
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