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Journal / Bioengineering / En forbedret fremgangsmåte for fremstillingen av…
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Bioengineering

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JoVE Journal Bioengineering
En forbedret fremgangsmåte for fremstillingen av type I kollagen fra hud
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En forbedret fremgangsmåte for fremstillingen av type I kollagen fra hud

Article DOI: 10.3791/51011-v • 05:17 min • January 21st, 2014
January 21st, 2014 •
1,2, 1, 1,2
1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

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Tradisjonelle prosedyrer for isolering av løselig type 1 kollagen (kol1) krever omtrent 10 dager fra start til slutt på grunn av lange buffer inkubasjoner og arbeidskrevende resuspensions av fibriller. Her beskriver vi et middel for å rense kol1 fra små dermal biopsier på mindre enn tre timer.

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Bioteknologi type 1 kollagen ekstracellulære matrise tissue engineering scaffoldprotein dermis corium
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