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Journal / Bioengineering / Samtidig kvantifisering av utvalgte kynureniner…
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Bioengineering

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JoVE Journal Bioengineering
Samtidig kvantifisering av utvalgte kynureniner analysert av flytende kromatografi-massespektrometri i medium samlet fra kreftcellekulturer
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Samtidig kvantifisering av utvalgte kynureniner analysert av flytende kromatografi-massespektrometri i medium samlet fra kreftcellekulturer

Article DOI: 10.3791/61031-v • 10:38 min • May 9th, 2020
May 9th, 2020 •
1, 1, 1
1Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin

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Beskrevet her er en protokoll for bestemmelse av fire forskjellige tryptofan metabolitter generert i kynurenin banen (kynurenine, 3-hydroxykynurenine, xanthurenic syre, 3-hydroksyantranisyre) i mediet samlet inn fra kreftcellekulturer analysert av flytende kromatografi kombinert med en enkelt quadrupole massespektrometri.

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Bioengineering Utgave 159 flytende kromatografi massespektrometri tryptofan metabolitter kynurenine 3-hydroxykynurenine xanthurenic syre 3-hydroksyantranisyre kreftceller prøve forberedelse
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