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JoVE Journal
Medicine

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优化高等级胶质瘤细胞培养从外科标本用于临床相关的动物模型和3D免疫化学
 
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优化高等级胶质瘤细胞培养从外科标本用于临床相关的动物模型和3D免疫化学

Article DOI: 10.3791/51088-v 12:25 min January 7th, 2014
January 7th, 2014

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Summary

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Click here for the English version.

在无血清神经球介质中分离出的高档胶质瘤手术标本的传播协议,为具有癌症干细胞表型的细胞进行选择。对于不能作为神经圈生长的标本,建议采取替代方案。描述了一种石蜡嵌入技术,用于维持免疫细胞化学的3D神经球结构。

Tags

医学,第83期,初级细胞培养,动物模型,神经系统疾病,肿瘤,胶质母细胞瘤,神经球,手术标本,长期自我更新
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