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Journal / Biology / BEST: Barcode Enabled Sekvensering av Tetrads
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BEST: Barcode Enabled Sekvensering av Tetrads

Article DOI: 10.3791/51401-v • 12:59 min • May 1st, 2014
May 1st, 2014 •
1, 1, 1, 1
1Pacific Northwest Diabetes Research Institute

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Barcode Enabled Sekvensering av Tetrads (BEST) erstatter manuelle prosesser for å isolere, forstyrre og avstands tetrads. BEST isolerer tetrads av fluorescens-aktivert celle sortering på agar plater, skiller sporene etter agitasjon med glassperler, og bestemmer hvilke tilfeldig kledd kolonier ble avledet fra samme opprinnelige tetrad ved hjelp av molekylære strekkoder.

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Genetikk gjær Tetrad gener DNA-sekvensering
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