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Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells
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Neuroscience
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JoVE Journal Neuroscience
Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

DOI:
10.3791/2639-v

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06:31 min

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March 06, 2011

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, , , ,
1Department of Neurosurgery,University of Florida, 2Department of Anatomical Sciences,Shiraz University of Medical Sciences, 3STEMCELL Technologies,Inc.

Chapters

  • 00:01Title
  • 00:44Introduction
  • 01:36Plating Cells
  • 03:58Replenishing medium
  • 04:50Representative results
  • 06:03Conclusion

Summary

Automatic Translation

Automatic Translation

This video protocol demonstrates how to discriminate and enumerate bona fide neural stem cells in a mixed population of neural precursor cells using the neural colony-forming cell assay.

Tags

Neural-Colony Forming Cell AssayAssayDiscriminateNeural Stem CellsNeural Progenitor CellsNeurosphere AssayIsolateExpandFrequencySerum-free Culture SystemTumorsNeurosphere FormationOverestimatesMixed PopulationEmbryonic BrainAdult Mammalian BrainCollagen-based Semi-solid AssayN-CFCAProliferative PotentialEnumerate NSC FrequencyColoniesFunctional CriteriaPrimary CellsCultured Cells

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