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July 03, 2017
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We present a method for quantifying growth phenotypes of individual yeast cells as they grow into colonies on solid media using time-lapse microscopy termed, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). Population heterogeneity of genetically identical cells growing into colonies can be directly observed and quantified.
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Cite this Article
Herricks, T., Mast, F. D., Li, S., Aitchison, J. D. ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth. J. Vis. Exp. (125), e55879, doi:10.3791/55879 (2017).
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