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Leverans av<em> In Vivo</em> Akut intermittent hypoxi i neonatal Gnagare Prime subventrikulära zonen-härledda neurala stamceller kulturer
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Delivery of In Vivo Acute Intermittent Hypoxia in Neonatal Rodents to Prime Subventricular Zone-derived Neural Progenitor Cell Cultures
DOI:

05:45 min

November 02, 2015

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Chapters

  • 00:05Title
  • 01:23Plethysmograph Chamber Set Up and Cycle Time Calibration
  • 02:54Acute Intermittent Hypoxia Administration and Subventricular Zone Stem/Progenitor Cell Culture
  • 04:33Results: Representative Neurosphere Development
  • 05:17Conclusion

Summary

Automatic Translation

I den här artikeln beskrivs metoden för att administrera korta perioder av intermittent hypoxi till postnatal dag 1-8 mus eller råttungar. Detta tillvägagångssätt effektivt framkallar en robust vävnadsnivå "priming effekt" på odlade neurala stamceller som skördas inom 30 min av hypoxi exponering.

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