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Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
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Bioengineering
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JoVE Journal Bioengineering
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

DOI:
10.3791/57769-v

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08:42 min

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September 15, 2018

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, , , , , , , ,
1Department of Bioengineering,University of California Los Angeles, 2Department of Bioengineering,University of Texas at Arlington, 3School of Optical and Electronic Information,Huazhong University of Science and Technology, 4Department of Biomedical Engineering,OSHU

Chapters

  • 00:04Title
  • 00:45Imaging System Setup
  • 03:32Imaging System Calibration
  • 05:07Sample Preparation
  • 05:48Adult Heart Imaging
  • 06:50Results: Light-sheet Fluorescence Imaging of the Murine Heart
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

This study uses a dual-sided illumination light-sheet fluorescence microscopy (LSFM) technique combined with optical clearing to study the murine heart.

Tags

Keywords: Light-sheet Fluorescence MicroscopyMurine HeartAir ChannelsGene TherapyCardiologyNeurologyPulmonologyContinuous Wave LaserBeam SplitterDual-sided IlluminationNeutral Density FilterBeam ExpanderPinholeBeam SplitterCylindrical LensAchromatic DoubletLens

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