Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

The main advantage of this technology is that it allows for rapid imaging of either the mouse heart. And it can be used to observe the presence of air channels after gene therapy. Although this method can provide insight into development cardiology, it can also be applied to other systems, such as neurology and pulmonology.

Generally, individuals will struggle with this technique because mounting an imaging of the adult mouse heart can be difficult, and it's different from other, traditional techniques, such as confocal and inverted microscopy. Place a continuous wave laser with three wavelengths of 405 nanometers, 473 nanometers, and 532 nanometers. Then, affix mirror one and align it with the mirror plane at 45 degrees to the beam.