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Immunology and Infection

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Analisi delle funzioni delle mastociti in vivo usando topi ' Mast Cell Knock-in'
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Analisi delle funzioni delle mastociti in vivo usando topi ' Mast Cell Knock-in'

Article DOI: 10.3791/52753-v • 09:07 min • May 27th, 2015
May 27th, 2015 •
1, 1, 1, 1, 1,2, 1
1Department of Pathology, Stanford University School of Medicine, 2Department of Microbiology & Immunology, Stanford University School of Medicine

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Descriviamo un metodo per la generazione di mastociti derivate in vitro, il loro innesto in topi carenti di mastociti, e l'analisi del fenotipo, dei numeri e della distribuzione delle mastociti innestati in diversi siti anatomici. Questo protocollo può essere utilizzato per valutare le funzioni delle mastociti in vivo.

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Immunologia e infezione Numero 99 c-kit fattoredi cellule staminali FcεRI immunoglobulina E modello di topo trasferimento adottivo immunologia allergia
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