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Orthotopisk Implantation og perifert immuncelle Overvågning i II-45 Syngene Rat mesotheliom Model
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Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Orthotopisk Implantation og perifert immuncelle Overvågning i II-45 Syngene Rat mesotheliom Model

DOI:
10.3791/53019-v

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09:31 min

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October 02, 2015

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, , ,
1Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney, 2Northern Blood Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney

Chapters

  • 00:05Title
  • 01:44In Vivo Cell Implantation
  • 03:46Tail Vein Blood Collection
  • 05:04Bead-based Immune Cell Profiling and Analysis
  • 06:42Results: Representative Circulating Immune Cell Counts from Rats with Mesothelioma
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Dannelsen af ​​en ortotopisk rottemodel for pleural malignt mesotheliom efter implantation af II-45 mesotheliom celler i pleurahulen af ​​immunkompetente rotter præsenteres. En flowcytometrisk metode til at analysere syv immuncelleundergrupper i disse dyr fra en 25 pi blodprøve er også beskrevet.

Tags

Keywords: Orthotopic ImplantationPeripheral Immune Cell MonitoringSyngeneic Rat Mesothelioma ModelImmune-based TreatmentsTumor MicroenvironmentFlow CytometryLymphocytesMonocytesNeutrophilsT-cellsB-cellsNatural Killer CellsNeutrophil To Lymphocyte Ratio

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