Journal
/
Medicine
/
Orthotopic Implantasjon og Peripheral Immune Cell Monitoring i II-45 syngeniske Rat Mesothelioma Model
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Orthotopic Implantation and Peripheral Immune Cell Monitoring in the II-45 Syngeneic Rat Mesothelioma Model

Orthotopic Implantasjon og Peripheral Immune Cell Monitoring i II-45 syngeniske Rat Mesothelioma Model

DOI:
10.3791/53019-v

•

09:31 min

•

October 02, 2015

•

, , ,
1Bill Walsh Translational Cancer Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney, 2Northern Blood Research Laboratory, Kolling Institute of Medical Research,Royal North Shore Hospital, University of Sydney

Chapters

  • 00:05Title
  • 01:44In Vivo Cell Implantation
  • 03:46Tail Vein Blood Collection
  • 05:04Bead-based Immune Cell Profiling and Analysis
  • 06:42Results: Representative Circulating Immune Cell Counts from Rats with Mesothelioma
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

Genereringen av en ortotopisk rottemodell av ondartet pleural mesothelioma ved implantering av II-45 mesothelioma celler inn i pleurahulen av immunkompetente rotter er presentert. En strømningscytometrisk metode for å analysere syv immuncelleundergrupper i disse dyrene fra et 25 mL blodprøve er også beskrevet.

Tags

Keywords: Orthotopic ImplantationPeripheral Immune Cell MonitoringSyngeneic Rat Mesothelioma ModelImmune-based TreatmentsTumor MicroenvironmentFlow CytometryLymphocytesMonocytesNeutrophilsT-cellsB-cellsNatural Killer CellsNeutrophil To Lymphocyte Ratio

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/53019?article_type=v

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy
check_url/53019?article_type=v

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
check_url/53019?article_type=v

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
check_url/53019?article_type=v

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
check_url/53019?article_type=v

Den Polyvinylalkohol Sponge Model Implantasjon
check_url/53019?article_type=v

Kirurgiske prosedyrer for en rotte modell av delvis Orthotopic levertransplantasjon med nedsatt arteriell Reconstruction
check_url/53019?article_type=v

Optimalisert System for cerebral perfusjon Monitoring i Rat Stroke Modell av intraluminal Middle Cerebral arterieokklusjon
check_url/53019?article_type=v

En "Patient-Like" ortotopiske syngeniske musemodell for leverkreft metastaser
check_url/53019?article_type=v

Isometrisk Contractility måling av musen hvem arterien bruker Wire Myography
check_url/53019?article_type=v

Reduserte komplikasjoner etter arteriell gjentilkobling i en rottemodell av ortotopisk levertransplantasjon

Read Article