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Journal / Bioengineering / Forbedring 2D og 3D Skin
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Bioengineering

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JoVE Journal Bioengineering
Forbedring 2D og 3D Skin
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Journal (Bioengineering)
Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip
 
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Forbedring 2D og 3D Skin

Article DOI: 10.3791/53642-v • 09:14 min • August 22nd, 2016
August 22nd, 2016 •
1,2, 2, 2, 1,2,3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 2Epithelial Biology Laboratory, Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

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Vi præsenterer en protokol for at opnå celle-afledte matricer rige på ekstracellulære matrixproteiner, hjælp makromolekylære Crowders (MMC). Derudover præsenterer vi en protokol, som inkorporerer MMC i 3D organotypisk hud co-kultur generation, hvilket reducerer dyrkningstid samtidig opretholde løbetid på konstruktionen.

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Bioengineering keratinocytter fibroblaster makromolekylær fortrængning 2D cellekultur 3D cellekultur hud organotypiske co-kulturer ekstracellulær matrix collagen type I collagen type IV collagen type VII, dermo-epidermal krydset
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