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Developmental Biology

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JoVE Journal Developmental Biology
Isolement des cellules embryonnaires murines Hemogenic endothéliales
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Isolement des cellules embryonnaires murines Hemogenic endothéliales

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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cellules souches et progénitrices hématopoïétiques (HSPC) proviennent de (hemogenic) des cellules endothéliales spécialisées au cours du développement, mais on sait peu sur le processus par lequel certaines cellules endothéliales spécifient pour devenir la formation du sang. Nous démontrons une méthode basée sur la cytométrie de flux permettant l'isolement simultané des cellules endothéliales hemogenic et HSPC de tissus embryonnaires murines.

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Developmental Biology numéro 112 endothélium hemogenic hématopoïèse définitive le développement vasculaire des embryons de souris FACS les cellules souches et progénitrices hématopoïétiques
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