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Journal / Developmental Biology / Isolement des cellules endocardiques et…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolement des cellules endocardiques et coronaires endothéliales du mur libre ventriculaire du cœur de rat
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Isolement des cellules endocardiques et coronaires endothéliales du mur libre ventriculaire du cœur de rat

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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Nous présentons un protocole pour l’isolement des cellules endocardiques et coronaires endothéliales endothéliales des coeurs de rat par la digestion séquentielle de tissu dans un tampon de digestion, la collection de cellules des cycles récurrents de centrifugeuses, et la purification de cellules utilisant des microbilles de CD31 anti-rat.

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Biologie du développement numéro 158 endocardium endothélium coronaire isolement cœur rat CD31
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