Transkriptions-Analyse von RNA Nascent FISH<em> In Vivo</em> Trophoblast-Riesenzellen oder<em> In Vitro</em> Kurzfristige Kulturen von Ectoplacental Cone Explantate
Trophoblast giant cells (TGCs) play a key role in the placenta to ensure a healthy pregnancy. We present a protocol for assessing the transcriptional status of genes in TGCs by nascent fluorescent in situ hybridization on cryostat sections of post-implantation embryos or short-term cultures of embryonic day 7 ectoplacental cones.