Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice

The overall goal of these surgical interventions is to measure the metabolic effects of sleeve gastrectomy and Roux-en-Y bypass in a mouse model. This method can help answer key question in the obesity and metabolism fields about the pathway on molecular mechanism involved in the metabolic gains observed after bariatric surgery. The main advantage of these techniques is that they stimulate the clinical consequences of bariatric surgery, as observed in human patient in a mouse model.

For a sleeve gastrectomy, after confirming the appropriate level of anesthesia by toe pinch, apply ointment to the mouse's eyes, and use an electric razor to shave the abdomen from sternum to pelvis. Cover the abdomen in plastic wrap, and place the animal in the supine position on the heating pad. Cut a window in the plastic wrap, and disinfect the skin with povidone iodine solution.