Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

M. Ang&#233;lica Bello; Yolanda Ruiz-Le&#243;n; J. Vladimir Sandoval-Sierra; Svetlana Rezinciuc; Javier Di&#233;guez-Uribeondo

doi:10.3791/55031

Journal

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Biology

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Microscopia elettronica a scansione (SEM) Protocolli per problematico Plant, oomicete, e campioni fungine

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

JoVE Journal
Biology

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JoVE Journal Biology

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Microscopia elettronica a scansione (SEM) Protocolli per problematico Plant, oomicete, e campioni fungine

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28,976 Views

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10:57 min

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February 03, 2017

DOI:

10.3791/55031-v

DOI:

10.3791/55031-v

•

10:57 min

February 03, 2017

•

28931 Views

M. Angélica Bello¹, Yolanda Ruiz-León², J. Vladimir Sandoval-Sierra³, Svetlana Rezinciuc⁴, Javier Diéguez-Uribeondo³

¹Biodiversity and Conservation Department,Real Jardín Botánico, CSIC ²Research Support Unit,Real Jardín Botánico, CSIC ³Mycology Department,Real Jardín Botánico, CSIC ⁴Division of Glycoscience, AlbaNova University Center,Royal Institute of Technology (KTH)

Summary

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.