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Journal / Developmental Biology / Isolement et caractérisation de cellules…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolement et caractérisation de cellules mésenchymateuses stromales du cordon ombilical humain et fœtale Placenta
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Isolement et caractérisation de cellules mésenchymateuses stromales du cordon ombilical humain et fœtale Placenta

Article DOI: 10.3791/55224-v • 07:06 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1,2, 1,2, 3, 1,2, 2,4, 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System

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Ici, nous présentons un protocole pour la dissection du cordon ombilical humain (UC) et l'échantillon de placenta fœtal en alignant le cordon (CL), la gelée de Wharton (WJ), de la jonction de cordon placenta (CPJ), et le placenta fœtal (FP) pour l'isolement et la caractérisation des cellules souches mésenchymateuses (CSM) en utilisant la technique de culture des explants.

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Developmental Biology numéro 122 périnatales le cordon ombilical de la jonction du cordon-placenta le placenta les cellules stromales mésenchymateuses des marqueurs de MSC des cellules multipotentes des marqueurs pluripotentes
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