Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Messung der G-Protein-gekoppelten…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Messung der G-Protein-gekoppelten Rezeptorsignalisierung über radioaktiv markierte GTP-Bindung
Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays
Journal (Biochemistry)
Direct Measurement of KDM1A Target Engagement Using Chemoprobe-based Immunoassays
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
Journal (Biochemistry)
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
 
Click here for the English version

Messung der G-Protein-gekoppelten Rezeptorsignalisierung über radioaktiv markierte GTP-Bindung

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Guanosintriphosphat (GTP) -Bindung ist eine der frühesten Ereignisse in der G-Protein-Coupled Receptor (GPCR) -Aktivierung. Dieses Protokoll beschreibt die pharmakologisch charakterisierende spezifische GPCR-Ligand-Wechselwirkungen durch die Überwachung der Bindung des radioaktiv markierten GTP-Analogs [ 35 S] Guanosin-5'-O- (3-thio) triphosphats ([ 35 S] GTPγS) in Antwort auf einen Liganden von Interesse.

Tags

Biochemie Ausgabe 124 Zelluläre Fraktionierung Membranpräparation GPCR Agonist Antagonist GTPγS-Bindung Pharmakologie μ-Opioidrezeptor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter