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Surveillance de la libération de calcium ER / SR avec le Ca ciblé
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Surveillance de la libération de calcium ER / SR avec le Ca ciblé

Article DOI: 10.3791/55822
May 19th, 2017 •
1, 1, 1, 1
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

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May 19th, 2017

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Nous présentons des protocoles pour l'application de notre indicateur de calcium codé génétiquement (GECI) CatchER + pour la surveillance des transitoires rapides de calcium dans le réticulum endoplasmique / sarcoplasmique (ER / SR) des cellules HEK293 et ​​C2C12 du muscle squelettique en utilisant une microscopie à fluorescence en temps réel. Un protocole pour la mesure et l'étalonnage in situ K d est également discuté.

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