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Journal / Biology / Otofajinin Kas Hücrelerinde Yerinde…
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Otofajinin Kas Hücrelerinde Yerinde İmmunofloresan Boyanması
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Otofajinin Kas Hücrelerinde Yerinde İmmunofloresan Boyanması

Article DOI: 10.3791/55908-v • 08:35 min • June 12th, 2017
June 12th, 2017 •
1,2, 3, 4, 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

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Aktif otofaji, Kas Kök Hücresi (MuSC) aktivasyonu için gerekli olan üretken kas rejenerasyonu ile ilişkilidir. Burada, LC3'ün in situ tespiti için bir protokol sunuyoruz, kontrol ve yaralı farelerdeki kas dokusu bölümlerinin MyoD pozitif MuSC'lerinde bir otofaji markörü.

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Hücresel Biyoloji Sayı 124 Otofaji kas kök hücreleri (uydular) kas rejenerasyonu LC3 MYOD kas dokusu kesitleri
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