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Biochemistry

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JoVE Journal Biochemistry
Kvantificering af overfloden og opladning niveauer af overførsel RNA'er i Escherichia coli
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
Journal (Biochemistry)
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Journal (Biochemistry)
Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
 
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Kvantificering af overfloden og opladning niveauer af overførsel RNA'er i Escherichia coli

Article DOI: 10.3791/56212-v • 10:34 min • August 22nd, 2017
August 22nd, 2017 •
1, 1, 1
1Department of Biology, University of Copenhagen

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Her præsenterer vi en metode til direkte måling af transfer RNA opladning niveauer fra renset Escherichia coli RNA samt en måde at sammenligne relative niveauer af transfer RNA eller nogen andre korte RNA, på tværs af forskellige prøver baseret på tilsætning af spike-i celler, der udtrykker en reference genet.

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Biokemi sag 126 Escherichia coli ribonucleic syrer ikke-kodende RNA RNA oprensning transfer RNA opladning niveau aminoacylation RNA kvantificering nordlige skamplet.
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