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Desensibilisering og genvinding af krebs-Photoreceptorer ved levering af en let stimulus
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Neuroscience
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JoVE Journal Neuroscience
Desensitization and Recovery of Crayfish Photoreceptors Upon Delivery of a Light Stimulus

Desensibilisering og genvinding af krebs-Photoreceptorer ved levering af en let stimulus

DOI:
10.3791/56258-v

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06:43 min

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November 09, 2019

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, , , , ,
1Departamento de Fisiología, Facultad de Medicina,Universidad Nacional Autónoma de México, 2Laboratorio Nacional de Canalopatías, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Chapters

  • 00:04Title
  • 01:01Experimental Setup
  • 02:40Eyestalk Isolation and Photoreceptor Impaling
  • 04:01Electrical Recording
  • 04:56Results: Recovery from Desensitization
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

En protokol til undersøgelse af desensibilisering og følsomhed nyttiggørelse af krebs-foto som funktion af cirkadiske tid er præsenteret.

Tags

Crayfish PhotoreceptorsDesensitizationSensitivity RecoveryCircadian TimeIntracellular Electrical RecordingsSingle Electrode Switched Voltage ClampNeurobiologyPhotoreceptor Electrical ActivityCell IsolationIntracellular MilieuMicropipette PullerPotassium Chloride SolutionElectrode ResistanceBridge ModeOffset CurrentCapacitive TransientsSuperfusion SystemEye Stock Isolation

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