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New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells
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JoVE Journal Engineering
New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells

New Application of an Atmospheric Pressure Plasma Jet as a Neuro-protective Agent Against Glucose Deprivation-induced Injury of SH-SY5Y Cells

DOI:
10.3791/56323-v

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06:34 min

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October 09, 2017

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, , , ,
1Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital,Capital Medical University, 2School of Physics,Beijing Institute of Technology, 3Beijing Key Laboratory of Central Nervous System Injury, Beijing Neurosurgical Institute, Beijing Tiantan Hospital,Capital Medical University, 4Beijing Key Laboratory of Translational Medicine for Cerebrovascular Disease, Beijing Tiantan Hospital,Capital Medical University, 5China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital,Capital Medical University

Chapters

  • 00:05Title
  • 01:24Acquisition of Jets
  • 02:27Preparation of SH-SY5Y Cells
  • 03:35APPJ Treatment of SH-SY5Y
  • 04:15Cell Viability Assay
  • 04:46Results: Atmospheric Pressure Plasma Treatent on Glucose Deprivation-induced SH-SY5Y Cells
  • 05:27Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for the neuroprotective application of low-dose atmospheric pressure plasma treatment on glucose deprivation-induced SH-SY5Y injuries.

Tags

Keywords: Atmospheric Pressure Plasma JetNeuro-protective AgentGlucose DeprivationSH-SY5Y CellsPlasma MedicineIschemic BrainCardiovascular SystemHigh Voltage Power AmplifierFunction Signal GeneratorHelium Flow RateSH-SY5Y Human Neuroblastoma-derived Cell LineTrypsinRPMI 1640 MediumFBSHemocytometer96-well Plate

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