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Journal / Developmental Biology / Immuno-fluoreszierende Etikettierung von…
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Developmental Biology

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JoVE Journal Developmental Biology
Immuno-fluoreszierende Etikettierung von Mikrotubuli und Centrosomal Proteine in Ex Vivo Darmgewebe und 3D In-vitro- Darm Organellen
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Immuno-fluoreszierende Etikettierung von Mikrotubuli und Centrosomal Proteine in Ex Vivo Darmgewebe und 3D In-vitro- Darm Organellen

Article DOI: 10.3791/56662-v • 09:51 min • December 13th, 2017
December 13th, 2017 •
1, 2, 1, 2, 1
1School of Biological Sciences, University of East Anglia, 2Norwich Medical School, University of East Anglia

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Wir präsentieren Protokolle zur Isolierung der intestinalen 3D-Strukturen aus in-Vivo -Gewebe und in-vitro- Keller-Matrix eingebettet, Organellen, und Detail verschiedene Fixierung und Färbung Protokolle für Immuno-Kennzeichnung von Mikrotubuli optimiert, centrosomal und Knoten Proteine sowie Handy-Marken einschließlich des Stammzell-Proteins Lgr5.

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Entwicklungsbiologie Ausgabe 130 organoide Darm Zellskelett Mikrotubuli EB1 CLIP-170 Ninein Centrosome Lgr5 Epithelien
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