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JoVE Journal Cancer Research
Utilizzando CRISPR/Cas9 Gene Editing per studiare l'attività oncogena di calreticulina mutante in cellule ematopoietiche Cytokine dipendente
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Utilizzando CRISPR/Cas9 Gene Editing per studiare l'attività oncogena di calreticulina mutante in cellule ematopoietiche Cytokine dipendente

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Genica mirata di editing con CRISPR/Cas9 ha notevolmente facilitato la comprensione delle funzioni biologiche dei geni. Qui, utilizziamo la metodologia CRISPR/Cas9 a modello calreticulina mutazioni in cellule ematopoietiche citochina-dipendente al fine di studiare la loro attività oncogena.

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Ricerca sul cancro problema 131 CRISPR Cas9 gene editing sgRNA calreticulina sequenziamento reporter
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