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Retrograd nevroanatomi sporing av Phrenic Motor Neurons i mus
 
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Retrograd nevroanatomi sporing av Phrenic Motor Neurons i mus

Article DOI: 10.3791/56758-v 09:05 min February 22nd, 2018
February 22nd, 2018

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Summary

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Her beskriver vi en protokoll for å identifisere phrenic motor neurons i mus etter intrapleural levering av fluorophore konjugert kolera gift delenhet beta. To teknikker sammenlignes for å injisere pleural hulrommet: transdiaphragmatic versus transthoracic tilnærminger.

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Nevrovitenskap problemet 132 Phrenic motor neurons retrograd merking intrapleural kolera gift delenhet beta mus nevroanatomi tracer
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