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Journal / Genetics / CRISPR/Cas9…
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Genetics

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JoVE Journal Genetics
CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏
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CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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本文介绍了一种快速 CRISPR/Cas9-mediated 基因在小鼠和人原发性造血细胞中的破坏的协议。Cas9-sgRNA ribonucleoproteins 是通过电穿孔与 sgRNAs 产生通过体外转录和商业 Cas9。在有限的时间和财务成本下实现了高编辑效率。

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遗传学 问题 134 CRISPR-Cas9 HSPCs 造血干细胞和祖细胞 敲出 ribonucleoprotein 基因编辑,
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