Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Interrupción génica altamente eficiente de las…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
Interrupción génica altamente eficiente de las células progenitoras hematopoyéticas murinas y humanas por CRISPR/Cas9
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Journal (Genetics)
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Journal (Genetics)
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
 
Click here for the English version

Interrupción génica altamente eficiente de las células progenitoras hematopoyéticas murinas y humanas por CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Un protocolo para rápido CRISPR/Cas9-mediado interrupción de genes en ratón y células hematopoyéticas primarias humanas se describe en este artículo. Cas9 sgRNA ribonucleoproteínas se introducen a través de electroporación con sgRNAs generado a través de transcripción en vitro y Cas9 comercial. Alta eficiencia edición se logra con tiempo limitado y costos financieros.

Tags

Genética número 134 CRISPR Cas9 HSPCs células madre y progenitoras hematopoyéticas knock-out ribonucleoproteína gene edición,
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter