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Journal / Genetics / Rottura del Gene altamente efficiente di cellule…
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Genetics

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JoVE Journal Genetics
Rottura del Gene altamente efficiente di cellule progenitrici ematopoietiche Murine ed umane di CRISPR/Cas9
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Rottura del Gene altamente efficiente di cellule progenitrici ematopoietiche Murine ed umane di CRISPR/Cas9

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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Un protocollo per la veloce rottura CRISPR/Cas9-mediata del gene nel topo ed emopoietici umani primari è descritto in questo articolo. Cas9-sgRNA ribonucleoproteine vengono introdotti tramite elettroporazione con sgRNAs generato tramite trascrizione in vitro e commerciale Cas9. Alta efficienza di editing si ottiene con tempo limitato e costi finanziari.

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Genetica problema 134 CRISPR-Cas9 HSPCs cellule staminali e progenitori emopoietici knock-out della ribonucleoproteina gene editing,
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