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Journal / Biochemistry / 真菌病原体液体荧光恢复法测定脂质指数黑穗病玉米…
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Biochemistry

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JoVE Journal Biochemistry
真菌病原体液体荧光恢复法测定脂质指数黑穗病玉米
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真菌病原体液体荧光恢复法测定脂质指数黑穗病玉米

Article DOI: 10.3791/57279-v • 10:22 min • April 3rd, 2018
April 3rd, 2018 •
*1,2, *3, 2, 1
1Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 2Facultad de Medicina, Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro
* These authors contributed equally

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在这里, 我们描述了一个获得脂滴指数 (LD 指数) 的协议, 以研究在高通量实验中培养的细胞中甘油三酯的动力学。LD 指数法是一种使用 BODIPY 493/503 的简便可靠的方法。这种检测不需要 dispendious 脂提取或显微分析。

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生物化学 问题 134 脂质指数 脂滴 担子 荧光恢复 BODIPY 493/503,黑穗病玉米
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