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JoVE Journal
Developmental Biology

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CRISPR 介导的小脑颗粒细胞在宫内电穿孔基因转移中的功能分析损失
 
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CRISPR 介导的小脑颗粒细胞在宫内电穿孔基因转移中的功能分析损失

Article DOI: 10.3791/57311-v 09:39 min June 9th, 2018
June 9th, 2018

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Summary

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传统的功能丧失基因的研究使用挖空动物往往是昂贵和耗时。基于电穿孔的 CRISPR 介导体细胞诱变是了解体内基因功能的有力工具。在这里, 我们报告了一个方法来分析的淘汰赛表型的小脑增殖细胞。

Tags

发育生物学 136 期 靶向基因传递,在子宫电穿孔 小脑 国产总值 CRISPR/Cas9 脑发育 脑肿瘤
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