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Journal
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Developmental Biology
/
CRISPR 介导的小脑颗粒细胞
在宫内
电穿孔基因转移中的功能分析损失
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using
In Utero
Electroporation-based Gene Transfer
Please note that all translations are automatically generated.
Click here for the English version.
CRISPR 介导的小脑颗粒细胞
在宫内
电穿孔基因转移中的功能分析损失
DOI:
10.3791/57311-v
•
09:39 min
•
June 09, 2018
•
Weijun Feng*
1
,
Lena Herbst*
2
,
Peter Lichter
,
Stefan M. Pfister
4,5
,
Hai-Kun Liu
,
Daisuke Kawauchi
4
1
Division of Molecular Neurogenetics
,
German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance
,
2
Division of Molecular Genetics
,
German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK)
,
3
Hopp-Children’s Cancer Center at the NCT Heidelberg (KiTZ)
,
4
Division of Pediatric Neurooncology
,
German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK)
,
5
Department of Pediatric Hematology and Oncology
,
Heidelberg University Hospital
Chapters
00:04
Title
01:30
In Utero
Electroporation
05:16
Preparation of Cryosections from Electroporated Cerebella
06:22
Immunostaining the Cerebellar Cryosections
07:38
Results: Immunostained Cerebella from
Rosa26-CAG-LSL-Cas9-P2A-EGFP
Mouse
08:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
传统的功能丧失基因的研究使用挖空动物往往是昂贵和耗时。基于电穿孔的 CRISPR 介导体细胞诱变是了解
体内
基因功能的有力工具。在这里, 我们报告了一个方法来分析的淘汰赛表型的小脑增殖细胞。
Tags
CRISPR
Loss Of Function Analysis
Cerebellar Granule Cells
In Utero Electroporation
Gene Transfer
Developmental Neuroscience
Neuro-oncology
Cerebellar Development
Brain Tumor Formation
Immunohistochemistry
Knock-out
Mouse
Plasmid
Fast Green
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