Abstract
An erratum was issued for Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum. There was a typo in the settings for Sutter P-87 in step 4.1.
Protocol
An erratum was issued for Larval RNA Interference in the Red Flour Beetle, Tribolium castaneum. There was a typo in the settings for Sutter P-87 in step 4.1.
Step 4.1 was updated from:
Pull borosilicate glass needles (B100-50-15, O.D.: 1 mm, I.D.: 0.5 mm, 15 cm length) by a needle puller.
NOTE: For Sutter P-87 or P-97, use the setting “Heat = 70, Pull = 45, Vel = 75, Time = 90” or follow Chapter 2 of the Pipette Cookbook: Adherent Cell, C. elegans, Drosophila, & Zebrafish– Recommended Programs. The optimal settings vary depending on the model of the needle puller. The setting for a generic Drosophila injection needle is a good starting point.
to
Pull borosilicate glass needles (B100-50-15, O.D.: 1 mm, I.D.: 0.5 mm, 15 cm length) by a needle puller.
NOTE: For Sutter P-87 or P-97, use the setting “Heat = 770, Pull = 45, Vel = 75, Time = 90” or follow Chapter 2 of the Pipette Cookbook: Adherent Cell, C. elegans, Drosophila, & Zebrafish– Recommended Programs. The optimal settings vary depending on the model of the needle puller. The setting for a generic Drosophila injection needle is a good starting point.
Disclosures
No conflicts of interest declared.
