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Journal / Genetics / Biotin-basierte Pulldown Assay, Validate mRNA…
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JoVE Journal Genetics
Biotin-basierte Pulldown Assay, Validate mRNA Ziele der zellulären miRNAs
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Biotin-basierte Pulldown Assay, Validate mRNA Ziele der zellulären miRNAs

Article DOI: 10.3791/57786-v • 11:00 min • June 12th, 2018
June 12th, 2018 •
1,2,3, 2, 2, 2
1School of Biotechnology, Kalinga Institute of Industrial Technology University, 21. Center for AIDS Health Disparities Research Department of Biochemistry and Cancer Biology, Meharry Medical College, 3Kalinga Institute of Industrial Technology University

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Dieser Bericht beschreibt eine schnelle und zuverlässige Methode zur Validierung von mRNA Ziele der zellulären MiRNAs. Die Methode verwendet, die synthetische biotinylierte gesperrt Nukleinsäure LNA-basierte MiRNA imitiert erfassen gezielt mRNA. Anschließend Streptavidin beschichteten magnetische Beads werden eingesetzt, um Pulldown das Ziel mRNA zur Quantifizierung von qPCR-Polymerase-Kettenreaktion.

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Genetik Ausgabe 136 MiRNA mRNA LNA biotinylierungen PCR 3' UTR
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