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JoVE Journal Biochemistry
Rensing og rekonstituering av TRPV1 for Spectroscopic analyse
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Rensing og rekonstituering av TRPV1 for Spectroscopic analyse

Article DOI: 10.3791/57796-v • 11:53 min • July 3rd, 2018
July 3rd, 2018 •
1, 2, 1,3, 1, 1
1Department of Physiology, University of Tennessee Health Science Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, 3CuriRX, Inc.

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Denne artikkelen beskriver bestemte metoder for å få biokjemiske mengder vaskemiddel-solubilized TRPV1 for spectroscopic analyse. Kombinert protokollene gir biokjemiske og Biofysiske verktøy som kan tilpasses for å lette strukturelle og funksjonelle studier for pattedyr ionekanaler i en membran-kontrollert miljø.

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Biokjemi problemet 137 forbigående reseptor potensielle vanilloid 1 TRPV1 rensing rekonstituering spin-merking EPR spektroskopi HJORT spektroskopi
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