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JoVE Journal
Biochemistry

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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
 

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Article DOI: 10.3791/59438-v 08:13 min April 9th, 2019
April 9th, 2019

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Summary

This method describes a robust and reproducible approach for the comparison of protein levels in different tissues and at different developmental timepoints using a standardized quantitative western blotting approach.

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Tags

Quantitative Western Blotting Protein Levels Tissues Development Fluorescent Total Protein Stain Internal Loading Control Snap-frozen Samples Protein Extraction Centrifugation Protein Concentration Bovine Serum Albumin Standards 96-well Optical Plate Absorption Measurement
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