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Journal / Medicine / 非放射性 l-azidohomoalanine…
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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究
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非放射性 l-azidohomoalanine 标记法分离原发性小鼠肝细胞对新生蛋白合成的研究

Article DOI: 10.3791/58323-v • 08:04 min • October 23rd, 2018
October 23rd, 2018 •
*1,2, *2, 2, 2, 2, 2, 2,3,4
1Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 2Division of Endocrinology, Cincinnati Children's Hospital Medical Center, 3Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 4Department of Pediatrics, College of Medicine, University of Cincinnati
* These authors contributed equally

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在这里, 我们提出了一个分离健康和功能的原发性小鼠肝细胞的协议。通过非放射性标记基底检测肝新生蛋白合成的说明, 帮助了解肝脏能量代谢稳态背景下蛋白质合成的机理。

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药物 140 期 原发性小鼠肝细胞 新生蛋白合成 AMPK 信号 鱼藤酮 l-Azidohomoalanine 非放射性方法
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