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Journal / Medicine / Generation af menneskelige 3D lungevæv kulturer…
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Generation af menneskelige 3D lungevæv kulturer (3D-LTCs) for sygdommen modellering
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Generation af menneskelige 3D lungevæv kulturer (3D-LTCs) for sygdommen modellering

Article DOI: 10.3791/58437-v • 05:47 min • February 12th, 2019
February 12th, 2019 •
1,2,3, 4,5,6, 4,5,6, 2,7, *1,2,3, *1,2,3,8
1Comprehensive Pneumology Center, Ludwig-Maximilians-Universität and Helmholtz Zentrum Munich, 2German Center of Lung Research (DZL), 3Translational Lung Research and CPC-M bioArchive, Helmholtz Zentrum München, Comprehensive Pneumology Center Munich DZL/CPC-M, 4Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, 5Wallenberg Center for Molecular Medicine, Lund University, 6Stem Cell Centre, Lund University, 7Asklepios Fachkliniken Munich-Gauting, 8Department of Medicine, Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado
* These authors contributed equally

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Vi præsenterer her, en protokol for forberedelse af Agarosen-fyldt menneskelige præcision-cut lunge udsnit fra resektion patient væv, der er egnet til at generere 3D lunge vævskulturer til model menneskelige lungesygdomme i biologiske og biomedicinsk undersøgelser.

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Medicin spørgsmål 144 3D vævskultur ex-vivo vævskultur præcision-cut lunge skiver PCLS 3D-LTCs lungesygdom sygdom dyremodeller menneskelige ex-vivo modeller lungefibrose
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