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Biochemistry

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Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter
 
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Et fluorescens udsving spektroskopi Assay af Protein-Protein interaktioner på celle-celle kontakter

Article DOI: 10.3791/58582
December 1st, 2018

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Summary December 1st, 2018

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Click here for the English version.

Denne protokol beskriver et fluorescens udsving spektroskopi-baseret tilgang til at undersøge interaktioner mellem protein mægle celle-celle interaktioner, dvs protein lokaliseret i celle vejkryds, direkte i levende celler. Vi leverer detaljerede retningslinjer på instrument kalibrering, dataopsamling og analyse, herunder rettelser til mulige artefakt kilder.

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