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Generation af kræft celle kloner til at visualisere Telomeric Repeat-holdige RNA TERRA udtrykt fra en enkelt Telomer i levende celler
 
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Generation af kræft celle kloner til at visualisere Telomeric Repeat-holdige RNA TERRA udtrykt fra en enkelt Telomer i levende celler

Article doi: 10.3791/58790
January 17th, 2019

Summary January 17th, 2019

Please note that all translations are automatically generated.

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Vi præsenterer her, en protokol for at generere kræft celle kloner indeholdende en MS2 sekvens tag på en enkelt subtelomere. Denne tilgang, lid MS2-normal god landbrugspraksis system, giver mulighed for visualisering af de endogene afskrifter af telomeric repeat-holdige RNA (TERRA) udtrykt fra en enkelt Telomer i levende celler.

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