Neuroscience
A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
利用功能近红外光谱进行超扫描实验
Chapters
Summary January 19th, 2019
Please note that all translations are automatically generated.
Click here for the English version.
本协议描述了如何进行 fnirs 超扫描实验和分析人脑同步。此外, 我们还讨论了挑战和可能的解决方案。
Transcript
通过同时测量两个或两个多个人的大脑活动,这种方法可以揭示社会互动背后的神经生物学机制。功能近红外光谱学(fNIRS)非常适合进行此类超扫描实验,因为它以高采样率测量脑血动力响应,并可应用于相对自然的环境中。虽然该协议显示如何同时进行成人-儿童的fNIRS记录,但它可以适应不同的迪亚迪星座,如浪漫伴侣和不同的实验任务。
首先选择相同大小或略大于参与者头围的帽大小。切割15个直径约15毫米的孔,以水平三五格栅格排列到两个原始EEG帽的每个前额区域。确保孔在任何方向上彼此之间 30 毫米,确保孔的中间列位于前额的中心。
为了使盖更舒适,并尽量减少压力标记,在探头插座和边缘之间的支架栅格内侧连接软泡沫材料。接下来,将空的三五探头支架栅格安装到每个经过修改的 EEG 盖上,使支架网格本身放置在盖内侧,支架插座粘在孔中。然后,轻轻地将探头插入网格上适当的支架插座,以便每个探头的第一个脊只安装在插座中,从而产生一次咔嗒声。
在最近的测量系统中打开探头集监视器窗口,并选择两个探头集,每个探头集排列在三五个网格中,一个用于参与儿童,另一个用于成人。确保两个大盖的探头布置与探头设置窗口中的布置一些。接下来,在测量前 30 分钟打开最近测量系统的激光二极管,使系统达到稳定工作温度。
在最近的测量系统中设置所有必要的选项。确保设备设置为事件相关测量,并且接收来自实验范式触发器所需的 RS232 串行输入处于活动状态。然后,通过启动作为心理物理工具箱扩展基础的技术计算软件,将当前目录设置为保存该范式文件夹,来准备实验范式。
最后,在计算机屏幕前放置两个下巴,以防止在实验中头部移动。首先护送两名参与者进入测试室并解释实验设置。然后,让学员在计算机屏幕前坐在一起。
调整下巴的高度,使两个参与者都舒适地坐着。提供指令并管理合作游戏和竞争游戏的实践试验。接下来,根据 10-20 系统测量和标记 Fpz 点,这是每个参与者头部的鼻和 inion 之间距离的 10%。
然后关闭激光,小心地将带探头的帽子放在参与者的头上。确保底部行的中间探头位于 Fpz 上,中间探针柱沿下垂参考曲线对齐。将纤维串放在连接到最近测量系统的支架臂上,以便它们松散地悬挂,无需与参与者或椅子接触,并且不会拉上盖。
接下来,将每个探头进一步推入其插座,直到探头外壳顶部中央的小白鼻子可见。然后,再次打开激光,并单击最近测量系统的探头集监视器窗口中的"自动增益"按钮来测试信号质量。如果通道标记为黄色,表示信号不足,请轻轻地将周围探头尖端下方的头发放在一边。
进行探头调整,然后再次单击"自动增益"按钮,查看信号质量是否有所改善或标记为绿色。一旦信号质量令人满意,在参与者的手上放置一条毛巾,这样他们就不能看到各自游戏伙伴的手部动作。然后,开始实验。
然后,在实验完成后,保存数据,通过单击"文本文件输出"按钮将原始光强度数据导出为文本文件。最后,清洁探头,探针架,下巴休息与民族,用温和的洗涤剂洗帽。作为大脑与大脑同步的度量,在相互作用的受试者的fNIRS信号之间计算小波一致性。
使用颜色图可视化小波相干系数,从蓝色、小或无相干性到红色,即强或最大相干。结果显示,在整个实验中,高频带的连贯性很强,直到周期长度约为一秒,这很可能是受试者的心脏节律导致的。此外,在两秒和八秒周期长度之间的较低频带中出现强相干性。
这种低频范围的一致性可能反映了两个受试者在任务期间大脑活动的同步。在尝试此过程时,必须仔细检查盖是否正确放置在参与者的头上,并确保良好的信号质量。该技术开发后,为社会神经科学领域的研究人员在各种不同任务中探索脑与脑同步,检查行为与个体特征及其关系的关系相关和关联铺平了道路。
Related Videos
You might already have access to this content!
Please enter your Institution or Company email below to check.
has access to
Please create a free JoVE account to get access
Login to access JoVE
Please login to your JoVE account to get access
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Please enter your email address so we may send you a link to reset your password.
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Your JoVE Unlimited Free Trial
Fill the form to request your free trial.
We use/store this info to ensure you have proper access and that your account is secure. We may use this info to send you notifications about your account, your institutional access, and/or other related products. To learn more about our GDPR policies click here.
If you want more info regarding data storage, please contact gdpr@jove.com.
Thank You!
A JoVE representative will be in touch with you shortly.
Thank You!
You have already requested a trial and a JoVE representative will be in touch with you shortly. If you need immediate assistance, please email us at subscriptions@jove.com.
Thank You!
Please enjoy a free 2-hour trial. In order to begin, please login.
Thank You!
You have unlocked a 2-hour free trial now. All JoVE videos and articles can be accessed for free.
To get started, a verification email has been sent to email@institution.com. Please follow the link in the email to activate your free trial account. If you do not see the message in your inbox, please check your "Spam" folder.