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Use of Drosophila S2 Cells for Live Imaging of Cell Division
JoVE Journal
Genetics
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JoVE Journal Genetics
Use of Drosophila S2 Cells for Live Imaging of Cell Division

Use of Drosophila S2 Cells for Live Imaging of Cell Division

DOI:
10.3791/60049-v

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00:06 min

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August 23, 2019

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, ,
1Department of Biology,University of New Mexico

Chapters

  • 00:04Title
  • 00:26Fluorescent Protein Expression Induction and Cell Imaging Preparation
  • 01:24Live Cell Imaging Program Setup
  • 02:34Image Dividing Cells
  • 04:31Results: Representative Live Imaging of Cell Cycle Defects Due to Mitotic Checkpoint Activation in S2 Cells
  • 05:50Conclusion

Summary

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Cell divisions can be visualized in real time using fluorescently tagged proteins and time-lapse microscopy. Using the protocol presented here, users can analyze cell division timing dynamics, mitotic spindle assembly, and chromosome congression and segregation. Defects in these events following RNA interference (RNAi)-mediated gene knockdown can be assessed and quantified.

Tags

Keywords: Drosophila S2 CellsLive ImagingCell DivisionMitosisFluorescent Protein ExpressionTime-lapse ImagingMulti-channel ImagingZ-stackPhotobleachingObjective Selection

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