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Journal / Immunology and Infection / Merkel cel Polyomavirus infectie en detectie
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Immunology and Infection

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JoVE Journal Immunology and Infection
Merkel cel Polyomavirus infectie en detectie
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Merkel cel Polyomavirus infectie en detectie

Article DOI: 10.3791/58950-v • 13:45 min • February 7th, 2019
February 7th, 2019 •
*1, *1, 2, 1
1Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, 2Laboratory of Cellular Oncology, National Cancer Institute
* These authors contributed equally

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Hier presenteren we een protocol om te infecteren primaire menselijke dermale fibroblast met MCPyV. Het protocol bevat Isolatievan dermale fibroblasten, voorbereiding van MCPyV virionen, virusbesmetting, immunofluorescentie kleuring en fluorescentie in situ hybridisatie. Dit protocol kan worden uitgebreid voor het karakteriseren van MCPyV-gastheer interacties en het ontdekken van andere celtypes ze door MCPyV.

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Immunologie en infecties probleem 144 Merkel cel polyomavirus isolatie dermale fibroblasten infectie collagenase IV CHIR99021 immunefluorescentie kleuring fluorescentie in situ hybridisatie in situ DNA-hybridisatie kettingreactie transcriptie replicatie
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