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JoVE Journal Biochemistry
Cel fractionering van U937 cellen in het ontbreken van snelle centrifugeren
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Cel fractionering van U937 cellen in het ontbreken van snelle centrifugeren

Article DOI: 10.3791/59022-v • 06:53 min • January 18th, 2019
January 18th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

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Hier presenteren we een protocol om te isoleren van het plasmamembraan, cytoplasma en mitochondria van U937 cellen zonder het gebruik van high-speed centrifugeren. Deze techniek kan worden gebruikt voor het zuiveren van subcellular breuken voor latere onderzoek van eiwit lokalisatie via immunoblotting.

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Kwestie 143 biochemie cellulaire fractionering mitochondriën membraan cytoplasma organellen centrifugeren U937
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